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filingDate 1986-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 1992-09-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 1992-09-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-0230869-B1
titleOfInvention Construction of an igg binding protein to facilitate downstream processing using protein engineering
abstract A recombinant DNA fragment (Z) coding for an immuno­globulin G binding domain related to staphylococcal protein A, characterized in that the methionine codon of said fragment has been replaced by a codon of another amino acid residue enabling expression of a methionine­free protein;a recombinant DNA sequence comprising at least two such fragments;a recombinant DMA sequence comprising such fragment preceded by a signal sequence and followed by a nucleotide sequence coding for the amino acid se­quence:Ala Gln His Asp Glu Ala;a recombinant DNA molecule comprising such recombinant DNA sequence and fused 3ʹ thereof at DNA level a pro­duction gene, said molecule having the ability to express a fused protein;a process for cleaving a fused protein expressed in a biological system by a such recombinant DNA molecule;a plasmid vector comprising said recombinant DNA molecule; anda bacterial cell harbouring such recombinant DNA molecule.
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