http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0219870-A3

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filingDate 1986-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 1988-11-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-0219870-A3
titleOfInvention Method for the detection of a polypeptide subunit in the presence of a quaternary protein containing the subunit
abstract A method for the determination of a free protein subunit nof a quaternary protein in a sample, which comprises: n(a) contacting a sample with a first immunological bind­ning partner which is or will be bound to a carrier, wherein the nfirst immunological binding partner binds eiptopic deter­nminants bindable only on the free protein subunit; n(b) incubating the components of step (a) for a period of ntime and under conditions sufficient to form an immune com­nplex between the free protein subunit, the first immunological nbinding partner, and the carrier; n(c) separating the carrier of step (b) from the sample; n(d) adding to the carrier of step (c), a detectably-labeled nsecond immunological binding partner, wherein the second nimmunological binding partner binds epitopic determinants nbindable on both the free protein subunit and the quaternary nprotein; and n(e) determining the detectably-labeled second im­nmunological binding partner in the carrier or in liquid phase.
priorityDate 1985-10-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

Incoming Links

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Total number of triples: 31.