http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0218480-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_97ddd4d1d0ebc8a7d2c8e7d214d08cf3 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-822 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0089 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 |
| filingDate | 1986-10-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3407a712e30498e15ca6619970abc8b5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fe7e578a7cd07816b265151bffb59c65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_373f5e95e367e7f5e63678ded7f27d2e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_953a43a59568125e013e97d380c4f483 |
| publicationDate | 1987-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-0218480-A2 |
| titleOfInvention | Novel superoxide dismutase and process for preparation thereof |
| abstract | A novel superoxide dismutase having the following properties: (a) action: the superoxide dismutase disproportionates two superoxide radicals to a hydrogen peroxide and a molecular oxygen; (b) substrate specificity: the superoxide dismutase acts on a superoxide radical: (c) optimum pH: the optimum a pH is about 8; (d) stable pH: the superoxide dismutase is stable at pH of 4.5 to 8.0 (when the superoxide dismutase is treated at 25°C for 60 minutes, the residual activity is at least 80%); (e) thermal stability: when the residual activity is measured after the heat treatment for 10 minutes at a pH of 7.8, the superoxide dismutase is stable at treatment temperatures of up to about 50°C, and the residual activity is 90% at a treatment temperature of 60°C, and 50% at a treatment temperature of 70°C; (f) molecular weight: the molecular weight is about 8.8 x 10⁴, as determined according to the gel filtration method; (g) absorption spectrum of ultraviolet and visible region: the maximum absorbance appears at about 280 nm, the minimum absorbance appears at about 250 nm and a shoulder appears at about 290 nm; (h) molecular extinction coefficient: the molecular extinction coefficient at 280 nm is about 1.45 x 10⁵ M⁻¹cm⁻¹; (i) inhibition: the activity is not inhibited by an addition of 1 mM potassium cyanide and exponential inactivation is caused with the lapse of time by an addition of 5 mM hydrogen peroxide; and (j) metal analysis: the superoxide dismutase contains 1.56 g-atoms of iron per mole of oxygen but does not contain copper and manganese; and a process for the preparation of the above-mentioned superoxide dismutase which comprises culturing a superoxide dismutase-producing strain belonging to the genus Norcardopsis in a culture medium to form and accumulate a superoxide dismutase in the culture medium, and recovering the superoxide dismutase from the cultured product. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0275202-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0275202-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0492447-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0628629-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5464614-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5536654-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/FR-2706465-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-607764-B2 |
| priorityDate | 1985-10-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 168.