| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a8e49a2b5f6d0ba32839687472b3a437 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6804
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-705
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 |
| filingDate |
1986-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_49286815432e869a07a3187f2d5cc8a3 |
| publicationDate |
1987-01-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-0209702-A1 |
| titleOfInvention |
Solid-phase hybridization assay using anti-hybrid antibodies |
| abstract |
A nucleic acid hybridization method for detecting a particular polynucleotide sequence present in a virus or cell contained in a test sample such as a biological fluid. The test sample is treated to release nudeic acids from the virus or cell of interest in single stranded form and to immobilize such nucleic acids on a solid substrate such as a filter membrane. Release of nucleic acids can be accomplished with alkali, lytic enzymes, detergents, and the like. The immobilized nucleic acids are contacted with a probe to form DNARNA or RNA RNA hybrids which are then detectable by binding of an anti-hybrid antibody reagent, preferably labeled. The method does not require the use of labeled probe and provides quantitative results due to the efficiency and stability of the immobilization of sample nucleic acids. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6025136-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5378604-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-4999290-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0288737-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-673813-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6228578-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/FR-2606154-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/FR-2606155-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9310263-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5212059-A |
| priorityDate |
1985-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |