http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0208647-A3

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filingDate 1986-07-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 1989-03-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-0208647-A3
titleOfInvention Immobilisation supports for chemical and physical processes and methods of their manufacture
abstract New immobilisation supports for use in physical and nchemical processes are produced from bird, animal or fish nbone by cleaning the bone of all tissue and finely dividing it to nan appropriate particle size. Bone consists of a mineral matrix nof calcium phosphate with organic connective collagen tissue nmaterial, principally the protein oseine, uniformly distributed ntherethrough. The oseine provides an ideal distributed site for nthe chemical attachment of bacteria, cells and organic enzyme ncatalysts, so that they are securely immobilised. It also con­nstitutes a suitable medium, for example, for affinity chromato­ngraphy in which complex molecules, such as antigens, form nspecific couples with support materials from which they can nbe washed out and decoupled for assay. The attachment may nbe by absorbtion, or by charge attraction, or may require a ncross-linking agent attachable between the oseine and the nsupported material. The particular material to be attached nmay require the use of a chelating agent or a buffering agent nto shield it from the action of the ionic calcium of the bone. A nparticularly economical source for the bone is the boney frac­ntion obtained from the mechanical separation of meat and nbone that is employed to recover meat from otherwise waste nproduct, e.g. chicken necks and backs unwanted by the nchicken fast food industry. Chicken bone is more porous than nanimal bone and thus particularly suitable for the purpose.
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Total number of triples: 32.