http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0208489-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f27c8bcbddcc6649bc70999e2ec7b00c |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-818 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-813 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-61 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-61 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 |
| filingDate | 1986-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cdeae82f99b9eb4499c4f325a948732a |
| publicationDate | 1987-01-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-0208489-A2 |
| titleOfInvention | High level microbial production of swine growth hormone |
| abstract | A high-density fermentation process for high yield production of a protein having swine growth hormone activity by transformant E. coli strains is described. The process employs transformant stains of E. coli containing a gene coding for a protein having swine growth hormone activity under the control of a bacteriophage lambda promoter-operator and a gene coding for the λc1857 temperature-sensitive repressor protein. In the initial growth period, the level of dissolved oxygen in the fermentation medium is maintained at about 20 % to 60 % of saturation and the temperature of the medium is maintained at a temperature at which the λ c1857 repressor protein is active. Production of the desired protein is then induced by raising the temperature of the medium to at least about 42°C. The temperature is then reduced to about 38°C to 41°C to optimize cell growth for the remainder of the induction period, during which the level of dissolved oxygen in the medium is maintained at about 10% to 40 % saturation, and the protein having swine growth hormone activity thus produced is recovered from the transformant cells. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0280407-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0248618-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-8805080-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9510620-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0280407-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5541086-A |
| priorityDate | 1985-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 174.