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filingDate 1986-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1986-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-0206687-A1
titleOfInvention A method for immobilizing enzymes, and in particular, to a method for converting cell bound enzymes into cell mass particles
abstract An improvement directed to the generation of particle form cell mass immobilized enzymes through cross-linking with polyazetidine prepolymer which comprises pretreating the enzymatically active microorganism cells in aqueous suspension with glutaraldehyde to effect partial cross-linking followed by dewatering and then admixing so-treated cell mass with the polyazetidine prepolymer followed by curing. n The dispersion of treated enzymatically active cell mass with the polyazetidine prepolymer may be sub-divided and shaped into granular form prior to curing, so that the immobilized enzyme product comprises cell mass enzyme granules of desired size and a controlled shape in high yield. n Glucose isomerase from a strain of the Streptomyces munnus cluster and tryptophan synthetase from a strain of E. coli are exemplified preferred embodiments.
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