| abstract |
@ A method of detecting a specific target polynucleotide sequence in a sample uses: a labelled first probe and a second probe, both probes to hybridise to different sequences of the target; and a solid phase carrying groups to bind the second probe. The method comprises contacting the sample under hybridising conditions with solutions of both probes, preferably in excess. Thereafter the mixture is contacted with the solid phase which is then washed. Target attached to the solid phase can be detected by means of the labelled first probe. The solid phase may be a dipstick carrying streptavidin to bind with the biotin groups attached to the second phase. |