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filingDate 1986-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1986-08-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-0190893-A2
titleOfInvention Test kit and method for the determination of streptococcus mutans
abstract The semi-quantitive detection of Streptococcus mutans from the oral cavity of a patient in vitro involves introducing saliva from a patient into a diluent solution to which has been added just before the saliva a predetermined powdered amount of bacitracin, and contacting the diluent solution with a sterile medium-coated paddle, then incubating the medium-coated paddle containing the diluent and test saliva, and thereafter semi-quantitively determining the amount of Streptococcus mutans in the test saliva by comparing the percent of colony density on the paddle with a standard Streptococcus mutans colony density chart. The amount of Steptococcus mutans in the oral sample is indicative of the patient's predispostion towards dental caries. n<??>A test kit for the determination of Streptococcus mutans includes an asceptic first vial (12) having a paddle (20) bearing a coating of medium (22) comprising agar and sucrose, a second asceptic vial (14) containing a buffered saline solution, a predetermined amount of bacitracin (28) e.g. in pellet form, a carbon dioxide generating composition (26), and standard Streptococcus mutans colony density charts (30).
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