| abstract |
in which A, and A 2 , identical or different, represent the hydrogen atom or a group SO 3 ⊖M⊕; M⊕ representing an alkali or ammonium cation, and R represents, with the nitrogen atom to which it is linked, a group derived from a hapten, an antigen with a molecular mass of less than 20,000 or a fragment d 'antibody.n n n To assay haptens or antigens of low molecular mass, a known amount of a compound 1 derived from the product to be assayed and recognized by the same antibody as the product to be assayed is introduced into the medium to be studied; introducing a given quantity of antibodies directed against the product to be assayed and the compound 1; the fluorescence polarization rate of the sample thus treated is measured, after incubation, and the concentration of the product to be assayed is determined by reference to standards. |