| abstract |
An improved, solid-phase analytical device for determining the presence or amount of a substance in a test sample, as well as an assay method using the devices is disclosed. The device comprises a porous, non-bilbulous fiber matrix impregnated with a hydrophobic polymer which forms a surface coating on at least a portion of the fibers of the matrix. The device also comprises a reagent capable of reaction with the substance in the sample; the polymer is capable of retaining the reagent within the matrix. The device of the invention is particularly advantageous for use in enzyme immunoassays. |