| Predicate |
Object |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-75
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-034 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-625
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-24
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-61 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-61
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-24
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 |
| filingDate |
1985-10-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
1992-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate |
1992-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-0177343-B1 |
| titleOfInvention |
Dna, cell cultures and methods for the secretion of heterologous proteins and periplasmic protein recovery |
| abstract |
Direct linkage of DNA encoding prokaryotic signals such as E. coli enterotoxin with DNA encoding mature eukaryotic proteins, followed by transformation and culture of bacterial hosts characterized by a periplasmic space, yields substantial amounts of periplasmic mature protein. Constitutively promoted gram negative bacterial mutants are transformed with vectors containing DNA encoding a secretable heterologous protein under the transcriptional control of a constitutively promoted promoter. Periplasmic proteins are recovered from transformed gram negative bacteria by a process comprising freezing and thawing the cells. Advantages are obtained by culturing the cells in phosphate-limiting media and by killing the cells prior to separation of periplasmic proteins. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8507008-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7678885-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8323902-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0015661-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7018982-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7771755-B2 |
| priorityDate |
1984-10-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |