| abstract |
A system and protocol for performing cytotoxicity studies including apparatus and methods for culturing the biopsied cells, for providing oxygenated nutrients, for removing cell-waste products, for introducing a fluorogenic substrate, for introducing cytotoxic agents including anti-cancer drugs, for measuring the released fluorescence and for measuring the released fluorescence and for measuring intracellular accumulation of fluorescein. Cytotoxicity can be determined by measuring fluorescence in the efflux of the vessel and concomitantly by direct photometric comparisons of the cells in the vessel before and after exposure to the cytotoxic agent. |