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filingDate 1985-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2004-09-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-0171142-B2
titleOfInvention Methods for producing proteins and transformed cells, and DNA constructs for correcting host cell deficiencies and their preparation and use
abstract Methods are provided for producing protein products in host cells and for selecting transformed cells comprising the step of transforming the host cell with a DNA molecule comprising a gene which complements a deficiency in the host cell. The host cell is a strain having a deficiency in a function necessary for normal cell growth. The gene in the DNA molecule, such as a plasmid, which complements the deficiency serves as a selection maker whereby the growth conditions for selection may comprise a conventional complex medium.
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