| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_81ff2fb1401923e9777f848215eb575f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_620939cea0c53e7263e5c04588ec80bb |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2201-0484
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2201-0446
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S436-821
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S436-80
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-973
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S436-808
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S436-807
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S436-809
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S436-805
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-966
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6419
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-963
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6421 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6452
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6458 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-25
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate |
1984-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c10215f068b63f924a9ede2dd605bf88 |
| publicationDate |
1985-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-0154743-A2 |
| titleOfInvention |
Method and apparatus for automated double fluorochromization analysis in lymphocytotoxicity testing |
| abstract |
An automated system for rapid sequential photometric analysis of a collection of double fluorochrome stained lymphocyte specimens, useful for antibody screening or lymphocytotoxicity analysis. The specimens are sequentially alternately irradiated with light of two distinguishable wavelengths, producing fluorescence at two distinguishable wavelengths. The fluorescent emission light intensity for each irradiation of each specimen is measured using a photometer and computer. The computer controls the synchronization of the irradiation through alternately selected condenser sets with the sequential movement of specimens Into the optical path of the irradiating and detected light, and calculates the ratio of the light intensities emitted from each specimen at the two selected fluorescent light wavelengths. These ratios are compared against a control ratio (for lymphocytotoxicity analysis) to classify the specimen. Also described is a method of preparing specimens for such analysis, which requires that a complement be added to the first staining solution after the latter is applied to the specimens, then this combination agitated, and then the second staining solution added and the specimen incubated. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0266881-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9310437-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2215838-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2196734-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2215838-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0266881-A2 |
| priorityDate |
1983-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |