| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a8e49a2b5f6d0ba32839687472b3a437 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-581
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-535
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-542 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-542
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-535 |
| filingDate |
1984-11-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3bfce29b0592308febc49ba35028a7fc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1eb2edea819f040d4da19debdc0578f6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0d3ac1ecd63dfbf24ab8d5e7c815889b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_49286815432e869a07a3187f2d5cc8a3 |
| publicationDate |
1985-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-0144744-A2 |
| titleOfInvention |
Specific binding assay based on enzyme cascade amplification |
| abstract |
The present invention provides specific binding assay methods and reagent systems for the quantitative or qualitative determination of an analyte in a liquid medium based on heterogeneous or homogeneous protocols. In particular, the invention relates to the use of specific binding assay techniques wherein the label is a participant in or a modulator of an enzymatic reaction whereby a first enzyme acts on a first substrate to produce a second enzyme. The second enzyme can be used to catalyze a detectable response or can act on a second substrate to produce a third enzyme. The label is chosen from the first substrate or a cofactor or modulator of the first enzyme. In one embodiment, the first substrate is a zymogen which when acted upon by the first enzyme produces a second enzyme. When the final enzyme product is used to catalyze the production of a detectable response proportional of the concentration of the analyte, amplification results providing a very sensitive assay. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9011523-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0685069-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5583001-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0685069-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5731158-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9011523-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0292170-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0292171-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-8909409-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0292171-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0292170-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5196306-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0174753-A1 |
| priorityDate |
1983-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |