| abstract |
@ Ice nucleation bacteria are modified in vitro to confer an ice nucleation deficient phenotype. Modification is accomplished by deletion, substitution, insertion, inversion, or transversion of a DNA segment within the gene locus responsible for the INA phenotype. By limiting such mutations to the particular gene locus, the modified microorganisms are genetically stable and free from random mutations which might adversely affect their competitive fitness. The modified microorganisms are useful for prevention of frost damage to susceptible plant hosts. |