| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_121b430efc3bbf2e965759ae24b5672b |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-75 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-75
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate |
1984-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7d1c26b26bb08b1f3b46dcea9407e712
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_83710e613b7e79d591aa1f506c28f545
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a72cd57cef4087ec60c2f3ace0106baa
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ccc9ff564797bc7e6b62365306e9a2ff |
| publicationDate |
1985-04-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-0138075-A1 |
| titleOfInvention |
A method for the generation and detection of enzyme variants with enhanced themostability and activity |
| abstract |
@ A method for transforming thermophilic microorganisms. A method for transforming thermophilic microorganisms is disclosed comprising: (a) forming a protoplast from a thermophilic microorganism; (b) introducing a plasmid chimera into the protoplast; and (c) causing regeneration of the cell wall of the protoplast. A novel regeneration medium is employed in the transformation method. This method involves the use of novel plasmid chimera. The plasmid chimeras include a newly discovered thermostable promoter. A method of isolating promoters capable of functioning at elevated temperatures is included. A method for selecting thermostable variants of gene products of cloned genes in thermophilic microorganisms is also disclosed. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6294358-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-8806624-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0118217-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0260299-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-8806624-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0260299-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5872238-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5958342-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0479396-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6001309-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6558633-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0896062-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5925732-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0896062-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5847105-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5786174-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6015880-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0118217-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6277334-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6350591-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6344327-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0283075-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0283075-A2 |
| priorityDate |
1983-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |