abstract |
A hybridoma which produces a monoclonal anti- peroxidase antibody having the following characteristics:n (a) of the sub-class IgG 1 ; (b) capable of binding to horse radish peroxidase to form a complex without significantly interfering with the catalytic activity of the enzyme, the complex containing a molar ratio of peroxidase to antibody of at least 1.78:1, preferably at least 1.96:1, and having an RZ value of at least 0.55, preferably at least 0.59; n may be obtained by fusion of spleen cells from a Balb/c mouse previously immunised with horse radish peroxidase and cells from the mouse myeloma cell line Sp2/0-Agl4. |