http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0136353-B1
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-948 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-34 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0037 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-005 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-07 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-077 |
filingDate | 1984-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 1989-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 1989-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-0136353-B1 |
titleOfInvention | Serum-free animal cell culture medium and method for the primary culture and obtention of cell lines using such medium |
abstract | Serum-free animal cell culture medium comprised essentially of: a) a synthetic base medium for the culture of animal cells; b) from 0.05 to 30 mueq/l of fatty acids or the esters thereof in proportions calculated with respect to the free acid of: 0.0155 to 24 mumole/l of palmitic acid; 0.0058 to 9 mumole/l of cis-palmitoleic acid; 0.0014 to 2 mumole/l of stearic acid; 0.0067 to 10 mumole/l of cis-oleic acid; 0.0178 to 27 mumole/l of cis-linoleic acid; and 0.0028 to 4 mumole/l of cis-linolenic acid, adsorbed on c) at least one lipophyl biopolymer in a quantity sufficient to provide for the dissolution of fatty acids or the esters thereof present in the medium. Said medium is particularly well adapted both to the primary culture and to the obtention of cell lineages, particularly epithelial cells of the liver or the kidney, of myelomatic cells and, particularly in the presence of growth hormons and/or factors, the hybrides thereof of the hybridoma type. |
priorityDate | 1983-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 28.