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filingDate 1983-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 1989-08-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 1989-08-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-0131020-B1
titleOfInvention Method for the volumetric differentiation of leukocytes
abstract A method and reagent system for differential determination of three-populations of leukocytes (lymphocyte, monocyte and granulocyte), using an automatic counting system. The reagent system includes a blood diluent and lysing reagent. The blood diluent is an osmotically balanced aqueous solution of ingredients at a preselected pH for maintaining erythrocyte morphology, blood cell stabilizing, buffering and bacteriostatic action. The lysing reagent is a mixture of an aqueous solution of quaternary ammonium salts, which lysing reagent is added to the diluted blood under more mild conditions of concentration and at a slower rate than is current practice in order to obtain an unexpected volumetric modification of at least one of the three-populations of leukocytes, whereby volumetric differential analysis can be accomplished.
priorityDate 1983-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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