http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0125867-A3

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filingDate 1984-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 1987-01-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-0125867-A3
titleOfInvention Assay systems using enzymes requiring NAD(P) as cofactor
abstract This specification discloses methods of detection or nmeasurement of an enzyme or of its specific substrate, and nsensors used in such methods. The present invention is con­ncenred with a multi-enzyme system, and the specification ndiscloses, as one aspect of the invention a method of assay in nwhich an electrode poised at a suitable potential is contacted nwith a system comprising a first enzyme, a cofactor linked nwith said enzyme and a mediator compound which transfers ncharge to the electrode from the first enzyme when its electri­ncal state is changed by reaction of cofactor material. n The cofactor may be NAD, NADP (both collectively refer­nred to herein as NAD (P) CAMP ATP GTP TTP CTP. n The specification particularly illustrates a method of nassay in which an electrode (1) poised at a suitable potential is ncontact with a system comprising a first enzyme El a nnicotinamide adenine dinucleotide compound N linked with nsaid enzyme El and a mediator compound F which transfers ncharge to the electrode from the first enzyme when its electri­ncal state is changed by a NAD(P) NAD(P)H reaction. The NAD ncompound may act as a "bridge" between the said enzyme//­nmediator system and further NAD utilizing enzymes E2.
priorityDate 1983-05-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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Total number of triples: 27.