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filingDate 1983-09-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 1991-01-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 1991-01-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-0118547-B1
titleOfInvention Method for the preparation of glucosyltransferase
abstract A method of preparing a purified glucosyltransferase (GTF) for use in immunization against dental caries, which method comprises: culturing a Streptococcus mutans in a medium containing glucose and dialyzable nutrients to form a mixture of culture cells and supernatant; recovering the supernatant by the removal of the culture cells; admixing the recovered supernatant with a water-insoluble, polymerized polysaccharide as solid particulate material for a period of time, to provide a GTF, solid particulate complex; recovering the GTF complex in solid particulate form by filtration; washing the solid GTF complex to remove unbound GTF and medium components; removing the GTF from the solid particulate material by a denaturing solvent; recovering the water-insoluble particulate material for reuse in the method; and recovering the GTF from the water-insoluble polysaccharide and purifying the recovered GTF.
priorityDate 1982-09-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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Total number of triples: 24.