http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0118547-B1
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-815 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-814 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1051 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-46 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10 |
filingDate | 1983-09-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 1991-01-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 1991-01-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-0118547-B1 |
titleOfInvention | Method for the preparation of glucosyltransferase |
abstract | A method of preparing a purified glucosyltransferase (GTF) for use in immunization against dental caries, which method comprises: culturing a Streptococcus mutans in a medium containing glucose and dialyzable nutrients to form a mixture of culture cells and supernatant; recovering the supernatant by the removal of the culture cells; admixing the recovered supernatant with a water-insoluble, polymerized polysaccharide as solid particulate material for a period of time, to provide a GTF, solid particulate complex; recovering the GTF complex in solid particulate form by filtration; washing the solid GTF complex to remove unbound GTF and medium components; removing the GTF from the solid particulate material by a denaturing solvent; recovering the water-insoluble particulate material for reuse in the method; and recovering the GTF from the water-insoluble polysaccharide and purifying the recovered GTF. |
priorityDate | 1982-09-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 24.