http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0108136-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2d9978c8f77db4c58666603ca189f025 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-539 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-582 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-539 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 |
filingDate | 1983-05-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b9d2d77cc1f3144090a3b0165f3f98af |
publicationDate | 1984-05-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-0108136-A1 |
titleOfInvention | COMPETITIVE IMMUNOFLUORESCENT TEST PROCEDURE AND SUITABLE TEST SET. |
abstract | Competitive immunofluorescence assays for the detection of antigens, in which the precipitation of immune complexes is carried out by means of a non-fluorescent and non-light scattering precipitant, such as a polyethylene glycol, and the resulting immunoprecipitate is dissolved with a non-fluorescent solvent of low ionic strength which keeps the pH of the solution substantially constant for a reading of the immunofluorescence intensity. The analyzes are performed by incubating the sample with a fluorescently labeled antigen, an anti-antigen antibody and a secondary antibody of the anti-antigen antibody, and by adding the precipitant to form an immunoprecipitate. The precipitate is separated by centrifugation, it is then dissolved in the solvent, and the immunofluorescence intensity of the solution is read using a fluorometer and compared to a standard curve. |
priorityDate | 1982-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 99.