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filingDate 1983-09-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1984-03-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-0103247-A2
titleOfInvention Method and device for determination of antithrombin-III
abstract Disclosed is a method and device for the quantitative determination of AT-III in mammalian blood plasma. The method involves contacting a plasma sample containing excess heparin with a 3 layered reagent strip comprising:n i. a first upper layer of a bibulous material containing thrombin and a buffer; ii. a second lower layer of a bibulous material containing a fluorogenic or chromogenic substrate capable of interacting with thrombin in a time related chemical manner; and iii. a bottom layer of water impermeable material. n The chemical change in the strip is determined as a function of time and this change is compared with that obtained in samples containing known amounts of AT-III.
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