http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0103081-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_be0bf5ca98d20b4a6ec28b58b085ca50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e121ec930f54ebe39cf423420be0b4a8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a957d1ce55c9f8fa087677d6aad1a73f http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4828398dbbbeae30197a3a1dd01ea7cb |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2502-02 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61M39-0247 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-163 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61D7-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61M1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61D7-00 |
| filingDate | 1983-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e9a61e4d46ad24ce893a4cf5ff33951c |
| publicationDate | 1984-03-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-0103081-A2 |
| titleOfInvention | Method of growing cells in vitro |
| abstract | An improved method and apparatus is disclosed for adding fluids to, or removing fluids from, a body part or organ of an animal which minimizes animal trauma and permits rapid, easy, repeated fluid or low viscosity gel transfers. The apparatus includes a tubular fluid-conveying element adapted for fixed connection to the animal, along with a flexible fluid conduit connected between the element and a specific internal body part or organ, such as a cow's uterus. In use, a syringe is employed to introduce or remove fluids from the body part, through the tubular element and connected conduit. The invention is especially adapted for introducing and recovering cell lines producing monoclonal antibodies or other biologically active products in large mammals, and facilitates monitoring of antibody production as well as administration of nutrients to enhance cell line growth. In preferred monoclonal antibody production procedures, the cells are initially educated through in vitro contact with ambient fluid of the selected organ (e.g., allantoic fluid from a pregnant host), whereupon the educated cells are inoculated and allowed to incubate in the host. It has been found particularly advantageous to employ the allantois of a pregnant mammal for antibody production, and some or all of the cell incubation period therein should occur after the onset of immunocompetency of the fetus. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9000412-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-750746-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0019937-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6228088-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/FR-2612394-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5332398-A |
| priorityDate | 1982-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 92.