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filingDate 1981-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1983-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-0076870-A1
titleOfInvention Process for the preparation of highly purified antihemophilic factor
abstract A process for the preparation of an aqueous composition of antihemophilic factor, having a concentration of about 20 to 45 units of antihemophilic factor per ml, a specific antihemophilic factor activity of at least 1 unit per mg protein, and being obtained from an aqueous solution of antihemophilic factor derived from human blood plasma, said aqueous solution having a concentration of about 5 to 15 units of antihemophilic factor per ml, and a specific antihemophilic factor activity of about 0.3 to 1.0 unit per mg protein, which comprises:n (a) effecting about a 2- to 10-fold concentration of the low concentrate solution, (13) (b) adjusting the pH of said concentrated solution of about 5.0 to 6.0, (16), (c) cooling the resulting solution to about 0 to 14°C, (17) (d) removing the precipitate which forms at said pH and temperature, (18) (e) further clarifying the solution from step (d), (21), and (f) adjusting the pH to about 7.0. (22)
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