| abstract |
Methods for the production of cDNAs representing viral sequences of RNA, such as double-fiber cDNA of the polio virus, its products, and its uses. Poliomyelitis virus cDNA is produced, for example, by reverse transcription of polio virus RNA and then by insertion of polio virus complementary DNA (cDNA) into bacterial plasmids by genetic manipulation techniques. The transformed bacteria are then cloned and cultured to produce replicated chimeric plasmids containing the cDNA poliovirus. Such a polio virus cDNA is useful for detection by testing for the presence of the polio virus and for the production of antibodies against the polio virus. It has been found that full-length polio virus cDNA is infectious, which means that it can be used to produce altered virus particles for vaccines. |