abstract |
A restriction enzyme having a specificity such that it can recognize a palindromic, hexanucleotide sequence 5'-GGCGCC-3', and can cleave the recognition sequence 5'-GGCGCC-3' between the two C's at the 3' ends, so as thus to generate DNA fragments with tetranucleotide long 3'- protruding ends, GCGC-3'. A method for production of this restriction enzyme employs strains belonging to Bifidobacterium breve, e.g. YIT 4006 having the Deposition No. FERM-P3906. |