http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0061761-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H19-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-00 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-48 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H19-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H19-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H19-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 |
| filingDate | 1982-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1988-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 1988-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-0061761-B1 |
| titleOfInvention | A method for preparing a mononucleotide-3'-phosphodiester-based substrate |
| abstract | A method for preparing a substrate capable of undergoing catalytic-induced hydrolysis of the phosphate ester at the 3 min -position to yield a species capable of being monitored spectrophotometrically or fluorometrically, comprises (a) blocking a mononucleoside of the formula: <CHEM> wherein B is a nucleotide base, and wherein the CH2OH group at the 4 min -position is either cis or trans to said base, with a silyl blocking member at both the 2 min -and 5 min -hydroxyls of said mononucleoside to form a 2 min -, 5 min -diblocked mononucleoside; and (b) forming a 2 min -, 5 min -diblocked mononucleotide 3 min -phosphodiester by bonding said 2 min -, 5 min -diblocked mononucleoside with a moiety selected from the group consisting of a chromophore or fluorophore; said silyl blocking member at the 2 min -hydroxyl being capable of at least essentially blocking medium-induced hydrolysis of the phosphodiester at the 3 min -position, and said silyl blocking member at least at the 2 min -hydroxyl being capable of being removed to provide a substrate characterized by the ability to undergo catalytic-induced hydrolysis of the phosphate ester at the 3 min -position to yield a species capable of being monitored spectrophotometrically or fluorometrically. The silyl blocking member at least at the 2 min -hydroxyl is removed so as to provide a substrate characterized by the ability to undergo catalytic induced hydrolysis of said phosphodiester to yield a species capable of being monitored spectrophotometrically or fluorometrically. |
| priorityDate | 1981-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 116.