http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0059182-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-087 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-04 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-23 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-04 |
| filingDate | 1981-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1984-11-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 1984-11-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-0059182-B1 |
| titleOfInvention | A process for isolating aminoacylase enzyme from a mammal kidney extract |
| abstract | An improved method for the isolation of aminoacylase enzyme from mammal kidneys. According to the invention an aqueous kidney extract, prepared in a manner known per se, is subjected to heat treatment at 60 to 80`C for 5 to 15 minutes, the heat treated mixture is centrifuged, 200 to 300 g/litre of a salt of the general formula K<ux>uA (wherein K stands for a monovalent cation, A is the anion of a mineral acid and x is equal to the valence of the anion) are added to the supernatant, the resulting suspension is centrifuged, the separated precipitate is dissolved in water, the solution is dialyzed against water, and, if desired, the dialyzed solution or a solution of the enzyme, separated from the dialyzate, in a buffer of pH 6.5 to 8.5 is subjected to anion exchange chromatography, finally, if desired, the active fractions obtained in the chromatographic step or a solution of the enzyme, precipitated from the active fractions with a salt of the general formula KxA, in a buffer of pH 6.0 to 8.0 is subjected to gel chromatography on a gel filter wherein the upper limit of the fractionation molecular weight is higher than 70,000, and the enzyme is precipitated from the active fractions with a salt of the general formula K<ux>uA as described above. The invention also relates to an immobilized aminoacylase enzyme consisting of a partially hydrolyzed A krilex P type acrylamide-N,N'-methylene-bis(acrylamide) copolymer and an aminoacylase enzyme attached to said copolymer by covalent bonds This immobilized enzyme preparation is prepared according to the invention so that a partially hydrolyzed Akrilex P type copolymer is treated with a carbodiimide compound which is soluble in water or soluble in an organic solvent at a temperature below 0`C, a solution of the aminoacylase with a pH of 6.5 to 8.5 is applied onto the resulting activated support, the resulting product is washed and then dried, if desired. By the improved method of the invention aminoacylase can be separated from mammal kidneys far more easily than before. The immobilized enzyme preparations according to the invention are of extremely high specific activity. |
| priorityDate | 1980-07-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 63.