| abstract |
Novel cointegrate plasmids pUC1012 and pUC1013, which are obtained by covalent linkage of the E. coli plasmid pBR322 to the Streptomyces espinosus plasmid pUC6, novel plasmids pUC1015 and pUC1022, which are obtained by restructuring plasmid pUC1012, and novel plasmids pUC1016 and pUC1023 which are obtained by restructuring plasmid pUC1013. These plasmids are useful as cloning vehicles in recombinant DNA work. For example, using DNA methodology, a desired gene, e.g. the insulin gene, can be inserted into one of the plasmids and the resulting plasmid can then be transformed into a suitable host microbe which, upon cultur-ing, produces insulin. |