http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0035121-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d5f0239be6386afbb67fea18159bee9c http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f06e5f747dc4682147fbcf34ad1b8d8f |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-04 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-04 |
| filingDate | 1981-02-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3d406ca09007ee72ff83e9f252a33126 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eafdcd6b7ab9bfa217afa771150d38ce http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_de1bfddc89f0a4e1593519a9f5ef0c95 |
| publicationDate | 1981-09-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-0035121-A2 |
| titleOfInvention | Process for preparing pearl-shaped biocatalysts comprising an extremely sensitive enzymatically active substance |
| abstract | The invention relates to a process for the production of biocatalysts with extremely sensitive enzymatically active substance in a further development of the process of patent application No. 80 2000 89.3 and is characterized in that the suspension or solution of an enzymatically active substance in the mixture (A) contains a cationic polyelectrolyte Contains concentration of 0.1 to 15 wt .-% and in the mixture (B), which contains the solution of a compound with polyvalent, opposite to the polyelectrolyte of the mixture (A), in the concentration range of 0.02 to 35%, in Temperature range from -5 to 80 ° C is injected to form pearl-shaped particles of 0.5 to 10 mm, and solidified with stirring in 5 min to 4 h, then filtered and introduced into a solution stabilizing the enzyme system and the network and be carried out in a damp state. The enzymatically active substance can consist of whole cells or cell fragments capable of reproduction or of an isolated enzyme or enzyme complex. Substances with sensitivity to multivalent cations or those with mechanical, temperature, drying sensitivity can be used. Mixture (A) as a cationic polyelectrolyte can contain a natural polymer or one after modification, such as a protonated chitosan or synthetic cationic polyelectrolyte, and in mixture (B) for crosslinking the cationic polyelectrolyte, the polymer of a negatively charged electrolyte or a polyvalent polyphosphate a polymer degree less than 15 or K4 (Fe (CN) 6) or K3 (Fe (CN) 6) in the concentration range from 0.006 to 1 mol / l. A phosphate buffer solution can be used as a solution for equilibrating the enzyme system and the network. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0244999-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0371408-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0371408-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0244999-A2 |
| priorityDate | 1980-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 30.