http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0024578-B1
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2326-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2326-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2326-96 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-96 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-96 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-92 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-18 |
filingDate | 1980-08-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 1983-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 1983-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-0024578-B1 |
titleOfInvention | Method of stabilizing an enzyme solution for use in total cholesterol determination, stabilized solution and test kit therefor |
abstract | Stabilized enzymes useful in the diagnostic assay of total cholesterol are prepared by dissolving a salt of cholic acid in a buffer solution providing a pH within the range of about 4 to about 9, to the solution is added a cholesterol esterase. The solution is then mixed with a polyhydroxy organic compound and TRITON-X-100. A cholesterol oxidase and a peroxidase are each dissolved in separate portions of buffer solution and introduced into the buffered solution containing the cholesterol esterase, 4-aminoantipyrine is then added to the solution. The resultant solution is a stabilized enzyme solution which can be used in the total cholesterol assay of a serum sample. The stabilized solution can be used in combination with a chromogen diluent solution which is made by dissolving phenol and TRITON-X-100 in water or a buffer solution. The combination of the stabilized enzyme solution and the chromogen diluent solution provides a solution which has utility in the spectro-photometric assay of total cholesterol. |
priorityDate | 1979-08-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 41.