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filingDate 1980-02-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1980-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-0016947-A1
titleOfInvention Process and reagent for the enzymatic determination of enzymatic substrates
abstract In a method for the enzymatic determination of an enzyme substrate in the presence of interfering substances which can react with the substrate itself or with an intermediate or end product of the indicator reaction, a certain amount of the substrate is first reacted with the reagent before adding the sample to be investigated until it has reacted and only then closes the sample to be determined. A reagent for performing this method contains a system for determining the substrate, which consists of at least one enzyme, at least one buffer substance and at least one indicator substance and optionally auxiliary substances, and also contains a certain amount of the substrate, for example glucose, uric acid or cholesterol , on the substrate determination capacity of the system for determining this substrate.
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