| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bc2b03342511abc88f60c63f2acd96d0 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y105-01003
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-003 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate |
1979-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d576e379d57b6ab2a7f1403e61d23019
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d1f1000da5fb55fc194b43f86b0bad02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_54856a3301a011742d953d3b054677c6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c4b1daa21fb7a5a50ec6313cfc2d15bf |
| publicationDate |
1980-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-0009930-A1 |
| titleOfInvention |
Recombinant DNA, method for preparing it and production of foreign proteins by unicellular hosts containing it |
| abstract |
Method and compositions are provided for producing proteins expressed by a DNA sequence free of proteins expressed by other DNA sequences where the DNA sequences comprise a functional recombinant DNA structure. The functional recombinant DNA structure is formed by annealing and/or ligating a first DNA sequence from a first source which encodes for a polypeptide; a second DNA sequence comprising a vector compatible with a unicellular organism which does not exchange genetic information with said first source, said vector having an intact replicon and a promoter for said first DNA sequence; and a third DNA sequence synthetically introduced between said first and second DNA sequences either prior to or concomitantly with annealing and / or ligating of said first and second DNA sequences encoding for a ribosomal binding site of said host, said third DNA sequence positioned adjacent a deoxyribonucleotide triplet encoding for methionine. The recombinant DNA structure is then employed in the transformation of a unicellular host to provide the host the capability of producing the polypeptide free of other polypeptides. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-4650761-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5795745-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-8605804-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0139076-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0139076-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/FR-2477134-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5424199-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-4506013-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H05268970-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0117058-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0117058-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6051426-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-4634677-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-4658021-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0022242-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5196194-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-4601980-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6475489-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5955307-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0157235-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0022242-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5324513-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5496711-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5525484-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/FR-2465631-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-4820642-A |
| priorityDate |
1978-10-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |