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filingDate 1979-08-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 1984-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 1984-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-0008969-B1
titleOfInvention Vaccine and method of making
abstract Culturing Streptococcus agalactiae in a nutrient broth containing at least 10 g l of glucose while maintaining the pH at 6-8 by the addition of alkaline material produces polysaccharide antigen including a fraction having a molecular size from 8-11 x 10<sup>5</sup>d. By employing a broth free from constituents having a molecular size above 10<sup>5</sup>, the antigens can be separated from the broth in high yield after harvesting the organisms. Vaccination of humans with Streptococcus agalactiae polysaccharide antigens having molecular sizes above 5 x 10' produces in selected individuals specific antibody levels above 200 µg ml of antisera; globulins fractionated from such antisera contain most of the antibody and can be used for passive immunization.
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