abstract |
The invention relates to a method for the determination of prothrombin in biological material, such as. B. blood plasma, by adding a defined amount of factor Xa, preferably human factor Xa, to the test solution and, after enzymatic cleavage of a thrombin substrate, for example a synthetic peptide-p-nitroanilide derivative, optionally measures a cleavage product such as p-nitroaniline photometrically. Furthermore, the invention relates to a reagent for carrying out the method and a method for producing a factor Xa preparation, preferably from human plasma. |