http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EA-202000149-A1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N23-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-49 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 |
| filingDate | 2020-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EA-202000149-A1 |
| titleOfInvention | METHOD FOR BACTERIA IDENTIFICATION FROM POSITIVE HEMOCULTURES BY MATRIX LASER DESORPTION IONIZATION TIME-OF-FLIGHT MASS SPECTROMETRY (MALDI-TOF MS) METHOD IN PATIENTS WITH BLOOD FLOW INFECTION |
| abstract | The invention relates to the field of medicine, in particular to microbiology with the possibility of application in patients in hematology, and can be used to identify bacteria from a positive blood culture by the method of matrix laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) in patients with a bloodstream infection. Preparation of positive blood cultures for the identification of microorganisms by the method of matrix laser desorption ionization time-of-flight mass spectrometry in patients with bloodstream infection is carried out by sample preparation of positive blood culture for analysis by the method of matrix laser desorption ionization time-of-flight mass spectrometry. At the same time, sample preparation of a positive blood culture is carried out by transferring it from a vial intended for cultivating microorganisms into a test tube with a separating gel and centrifuged at 3000 rpm for 10 minutes. The resulting supernatant is mixed, transferred to a microcentrifuge tube and centrifuged at 3000 rpm for 2 min. After that, the supernatant is transferred to a second microcentrifuge tube and centrifuged at 13,000 rpm for 2 minutes. The supernatant is removed, deionized water is added to the precipitate and mixed on a vortex, and then 96% ethyl alcohol is added and mixed on a vortex again, after which it is centrifuged at 13000 rpm for 2 min. Then the alcohol is removed and centrifuged at 13,000 rpm for 2 min, and the remaining alcohol is removed, leaving the microcentrifuge tube with the lid open until the alcohol has completely evaporated. Next, the protein extract is extracted with formic acid and acetonitrile, stirring the mixture on a vortex and centrifuging at 13,000 rpm for 2 minutes. The resulting supernatant is applied to the target of the mass spectrometer, after drying, covered with a matrix, and microorganisms are identified by the method of matrix laser desorption ionization time-of-flight mass spectrometry in patients with bloodstream infection. The technical result consists in reducing labor intensity due to the introduction of a smaller amount of reagents and reducing the time for preparing positive blood cultures, which ensures high accuracy in the identification of microorganisms from a bloodstream containing bacteria. |
| priorityDate | 2020-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 20.