http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EA-201900232-A1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0735 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-00 |
| filingDate | 2019-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EA-201900232-A1 |
| titleOfInvention | METHOD FOR OBTAINING PLURIPOTENT STEM CELLS PRODUCED BY TRANSFER OF SOMATIC CELL NUCLEI |
| abstract | The invention relates to biotechnology, in particular to genetic engineering, and can be used to obtain pluripotent stem cells produced by the transfer of nuclei of somatic cells. The technical result is to obtain pluripotent human stem cells, endowed with improved epigenetic reprogramming. This is achieved by the fact that in the method of obtaining pluripotent stem cells produced by the transfer of nuclei of somatic cells, according to the invention, the donor oocyte is enucleized by removing the metaphase II spindle without reducing the level of the maturation factor and with the formation of a cytoplast; carry out the interaction of the donor nucleus with the extract of HVJ-E, followed by the interaction of the cytoplast with the donor nucleus to form an SCNT embryo; treated with a protein phosphatase inhibitor comprising caffeine, an oocyte and / or cytoplast and / or a donor nucleus and / or an SCNT embryo; effecting at least one electroporation pulse on the SCNT embryo to obtain an activated SCNT embryo; cultivating the activated SCNT embryo in the first medium, then in the second medium, then in the third medium with the formation of a blastocyst; cultivate blastocysts on the feeder layer, followed by selection of cells with embryonic stem cell morphology. |
| priorityDate | 2019-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
| Predicate | Subject |
|---|---|
| isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID419509525 http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID2519 |
Total number of triples: 12.