http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EA-201600529-A2
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-59 |
| filingDate | 2016-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EA-201600529-A2 |
| titleOfInvention | METHOD FOR IDENTIFYING PROTEIN IN BIOLOGICAL FLUIDS |
| abstract | The invention relates to the field of medicine and can be used in clinical diagnostic laboratory practice for carrying out analyzes on the protein content in biological fluids, in particular in human saliva, in order to diagnose various diseases and monitor the course of treatment. The essence of the method - the method is based on the fact that, mix the reagent solution with a sample of biological fluid, incubate the resulting mixture with stirring for 10 minutes and determine the protein concentration after incubation by measuring the optical density of the mixture relative to the blank sample at a wavelength of 578-620 nm and the subsequent calculation according to the calibration schedule, while human saliva is used as a sample of biological fluid, to obtain a reagent used for mixing with a sample of biological fluid, rimenyayut pyrogallol red mixture was 0.06 mmol / l of sodium molybdate 0.04 mol / l and succinate buffer at pH 2.5. To obtain a mixture of reagent solution with a sample of biological fluid, 1 ml of the prepared reagent is added to the tube before incubation, 20 μl of saliva and 3 ml of distilled water are added, and the optical density of the mixture after incubation is measured against a single sample in a cell with a thickness of 10 mm waves 598 nm for no more than 30 minutes, while the blank sample is prepared as a mixture of reagent with distilled water in the same volume as in the mixture of reagent with a sample of biological fluid, with the same operations This, and the determination of the protein concentration is carried out according to a calibration curve, which is preliminarily constructed at no less than five points, using a standard protein solution containing 70% albumin and 30% globulin in concentrations from 0.2 to 4.00 g / l. The invention achieves the required technical result, which consists in expanding the scope. |
| priorityDate | 2016-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 22.