http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EA-201600528-A3
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-28 |
| filingDate | 2016-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EA-201600528-A3 |
| titleOfInvention | METHOD FOR DETERMINING SIAL ACIDS IN BIOLOGICAL LIQUIDS |
| abstract | The invention relates to the field of medicine, namely clinical laboratory diagnostics and can be used to determine the quantitative content of sialic acids in biological fluids. The essence of the invention is a method for determining sialic acids in biological fluids is that, prepare the initial mixture, including a sample of saliva, to prepare the initial mixture contribute to the tube 1 ml of a solution of trichloroacetic acid with a concentration of 100 g / l, 2 ml of distilled water and 0, 3 ml of saliva and mix them thoroughly, then the tube with the initial mixture is placed in a boiling water bath and held there for 5 minutes, then cooled to room temperature, then centrifuged for 10 minutes at a rate of rotate the centrifuge at 2500 rpm and then take 2 ml of supernatant from it, add 0.4 ml of acetic acid sulfate mixture and place it again in a boiling water bath and hold it for 15 minutes, then cool to room temperature, add 2 ml of distilled water and measure the optical density of the resulting solution against a blank sample, which is prepared as a solution of sulfuric acid in glacial acetic acid, with a green filter (500 - 560 nm) in a cell with a layer thickness of 10 mm, and determining the concentration of sialic acids carry out according to the calibration schedule which is built previously not less, than on five points, using standard solution of N-acetylneuraminic acid in concentration from 0,050 to 1,00 mmol / l. The technical result, which is implemented using the proposed method, is to simplify the method, increase efficiency and reduce material consumption. |
| priorityDate | 2016-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 21.