| abstract |
The application describes a new method for producing glycoproteins using a mammalian cell culture in which the sialic acid content of the glycoprotein produced is controlled in a wide range of values, varying the medium for cell culture. The invention relates to methods in which the content of sialic acid in the glycoprotein is modified by changing the parameters of cell cultivation, affecting the specific productivity of cells. Preferred embodiments of the invention include cell culture methods in which, during the protein production phase, the cell culture controls the osmolality of the cell culture, as well as the concentration of the transcription enhancer. In addition, the invention relates to new drugs, including soluble tumor necrosis factor type 1, immunoglotulin G, and their use for the treatment of inflammatory diseases or disorders associated with the immune system. The international application was published along with an international search report. |