| abstract |
According to the invention, endoglycosidase is proposed, designated EndoS49 and having the amino acid sequence of SEQ ID NO: 1. EndoS49 was isolated from Streptococcus pyogenes strain NZ131, it is a homologue of EndoS. EndoS49 has a specific endoglycosidase activity against native IgG and cleaves a wider range of Fc glycans than EndoS. Its mutant was obtained by replacing glutamic acid at position 186 of SEQ ID NO: 1; this mutant does not possess endoglycosidase activity, but is able to bind to IgG. Disclosed are methods using EndoS49, its deletions and said mutant, especially for assessing IgG glycosylation or IgG isolation. |