| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6ebfd716a6d868cec0fdcf2af2480107 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-10351
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-10343
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-10321 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-861
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M3-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-861
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M3-06 |
| filingDate |
2011-02-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aa80516bb4311b8b3ffb041308b26465
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9b8537883afa183e4e7b21e12a558865 |
| publicationDate |
2016-07-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EA-023816-B1 |
| titleOfInvention |
METHOD OF PRODUCING VIRAL PARTICLES Ad26 |
| abstract |
The invention relates to a method for producing at least 1 × 10 viral particles (VP) / ml of E1-deficient serotype 26 adenovirus (rAd26), the ratio of the absolute number of virus particles to infectious particles VP / IU is less than 20: 1, and the method includes a) cultivating PER.C6 cells in suspension using a perfusion system; b) infection of these cells by rAd26 at a density of 10 × 10 to 16 × 10 viable cells / ml; c) further culturing infected cells using a perfusion system to propagate said rAd.26 until a concentration of at least 1 × 10 viral VP / ml particles is reached and d) collecting viral particles of said rAd26. The invention also relates to the use of a bioreactor in said method, where said bioreactor has a working volume of from 2 to 1000 liters and contains culture medium, PER.C6 cells and rAd26 virus particles at a concentration of at least 1 × 10VP / ml. |
| priorityDate |
2010-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |