http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EA-022782-B1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f3166d2820b5872ab84d235e5fae1d45 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-535 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-535 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-67 |
| filingDate | 2007-03-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c0b790bc6874c5559ef34130fd397fd0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b53bbcc11a78424b6d9609619105ba48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8e728a13810819d8d4a7ae219a61f087 |
| publicationDate | 2016-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EA-022782-B1 |
| titleOfInvention | METHOD OF OBTAINING H-CSF HUMAN |
| abstract | The invention relates to a method for producing granulocyte colony-stimulating factor (G-CSF) with a high volumetric yield (5.8-8.3 g / l) under non-cyclic cultivation with feeding of bacterial cells transformed with a plasmid with a human G-CSF gene, in media containing 5-10 g / l of thiamine, 60-180 mM of potassium ions, as well as 60-300 mM of sodium ions or 150-250 mM of magnesium ions. Growing and increasing the biomass of these cells is carried out until the optical density of the medium is approximately 50 units (at a wavelength of 600 nm). Next, isopropyl thiogalactoside IPTG is added to induce the expression and production of G-CSF. The method allows to obtain G-CSF with a high volumetric yield due to the maintenance of high stability of the plasmid using a simple and stable process. |
| priorityDate | 2006-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 53.