http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EA-020657-B1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2f360e5132eaa76c771ceb9ad5b4656a |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-19 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1024 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate | 2008-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dd65221482d39537c86eb1e13479968e |
| publicationDate | 2014-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EA-020657-B1 |
| titleOfInvention | SPECIALIZED MULTI-SITE COMBINATOR ASSEMBLY |
| abstract | The present invention relates to a new method for producing a variety of modified polynucleotides having different combinations of different mutations at multiple sites by means of a specialized multi-site combinatorial assembly, comprising adding at least two or at least three primers to a double-stranded template polynucleotide in one reaction mixture, where the primers are not overlap and where each of the primers contains at least one mutation that is different from other primers, where at least e one primer is a direct primer capable of annealing with the matrix negative chain, and at least one primer is an inverse primer capable of annealing with the matrix plus chain, and exposing the reaction mixture to a polymerase addition reaction to obtain a plurality of completed modified polynucleotides from at least one three primers. The method can be carried out without the use of a ligation step prior to cell transformation with completed modified polynucleotides. Many completed modified polynucleotides can be treated with an enzyme before cell transformation to destroy the template polynucleotide. |
| priorityDate | 2007-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 364.