http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EA-018116-B1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b97d4f6f618c15f9ce7dd420b4cb135b |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K47-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-505 |
| filingDate | 2011-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7a65235fb009362b9be1302d5cf27f05 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d12ac4d0da49b17346833c6422ee2929 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2472b7e1ebbed7e89243d05a0c9c2491 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3abf85e66245390dc7b8734c57b9a05a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9e63df5b4acfb633f7decfaa44f27f80 |
| publicationDate | 2013-05-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EA-018116-B1 |
| titleOfInvention | Method of producing covalent conjugate of polyethylene glycol with erythropoietin |
| abstract | The invention relates to a method for producing a covalent conjugate of polyethylene glycol with erythropoietin, characterized in that: a) the free hydroxyl group of monomethoxy polyethylene glycol is acylated with nitrobenzoic acid halide in anhydrous pyridine; b) the nitro group of the nitrobenzoic acid methoxy polyethylene glycol ester obtained is reduced with a chromium (II) salt; c) aminobenzoic acid methoxypolyethyleneglycol ether obtained is diazotized with alkali or alkaline earth metal nitrite in an aqueous or aqueous-organic medium or organic nitrite in a polar organic solvent medium, followed by removal of excess nitrite and obtaining an activated pegylating agent; d) the activated pegylating agent is introduced into the azo coupling reaction with erythropoietin in an aqueous or aqueous-organic medium with a pH from 7.0 to 9.5; e) a mixture of pegylation products is separated by ion exchange chromatography to obtain mono-pegylated erythropoietin. Technical result: expansion of the spectrum of erythropoietin with an increased circulation time in the bloodstream. |
| priorityDate | 2011-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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