http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EA-011823-B1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c834eb81d6424e6d4f4cefe9cd06e34d |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K2039-53 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-90 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-61 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2004-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_298eea94b0828a642a58acaac988324a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cc778fdce1e3b72bdb66769bf6764873 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_912e10f142308703e8ef58e53ae409f1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_30ad25f371a7fa7c1adbed57c6566771 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3a8ffd26dda2a6703ff4b4bb6433cdc9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8acfba52224ab8f4588bbe052bc322a3 |
| publicationDate | 2009-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EA-011823-B1 |
| titleOfInvention | BACTERIAL SYSTEM OF BREEDING AND ITS APPLICATION |
| abstract | A selection system containing a bacterial cell deficient in the araD gene encoding L-ribulose-5-phosphate-4-epimerase (EC 5.1.3.4.), Into which a vector carrying the araD gene encoding active L-ribulose is inserted as a marker for selection -5-phosphate-4-epimerase. A method for selecting bacterial cells transformed with a vector containing the araD gene encoding active L-ribulose-5-phosphate-4-epimerase as a selection marker and a gene of interest, the method comprising inserting the vector into a host cell deficient in the araD gene, encoding L-ribulose-5-phosphate-4-epimerase, and growing the obtained cells in a growth medium containing arabinose. A non-antibiotic selection marker makes this system suitable for the production of therapeutic agents. The araD gene is not essential for host growth, but its manipulation affects growth in some selective conditions. Deletion of araD leads to the accumulation of a substance that is toxic to the host, but not to humans. The araD gene is relatively small, and therefore a small vector can be constructed that requires less energy for replication and leads to increased growth rates and yields. |
| priorityDate | 2003-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 427.