| abstract |
The present invention is based on the creation of a double promoter system (preferably the pol I-pol II RNA system) for efficient intracellular synthesis of viral RNA. The resulting system, based on the minimum number of plasmids, can be used to synthesize RNA of the virus, preferably viruses with the genome represented by negative single-stranded RNA. The viral product of the system is formed when the plasmids of the system are introduced into the corresponding host cell. One embodiment of the invention is to produce attenuated reassorted influenza viruses for use as antigens in vaccines. Reassorted viruses obtained by cotransfection of plasmids may contain genes encoding surface glycoproteins - hemagglutinin and neuraminidase from the influenza virus, which currently infects the population, and internal genes from attenuated influenza virus. One of the advantages of the present invention is its versatility; the system can be quickly and easily adapted to synthesize a weakened variant of any virus RNA. Attenuated or inactivated RNA viruses obtained according to the present invention, if necessary, vaccination can be administered to the patient by any of several methods, including intranasally or intramuscularly. |